T0198
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 | Immunoblotting Total cell extract of PC-12 was separated on SDS-PAGE and blotted with Monoclonal Anti-β-tubulin (Cat. No. T0198). The antibody was developed with Goat Anti-Mouse IgG, alkaline phosphatase conjugate (Cat. No. A2179) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody |
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T0198
Sigma
Monoclonal Anti-β-Tubulin antibody produced in mouse
~1 mg/mL, clone D66, purified immunoglobulin, buffered aqueous solution
| MDL number: | MFCD03792414 |
Description
| Application | Can be used in studies involving the inhibition of flagellar motility in permeabilized sperm models. |
| Immunogen | sea urchin (Lytechinus pictus) sperm axonemal proteins. |
| Specificity | Recognizes all mouse brain β-tubulin isoforms (β and β′ subpopulations), and sea urchin β2-tubulin. The epitope recognized by the antibody is localized in the C-terminal region of β-tubulin (amino acids 427-432 in sea urchin β2-tubulin). |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | D66 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~50 kDa |
| species reactivity | Chlamydomonas reinhardtii, canine, rabbit, sea urchin (Lytechinus pictus and Paracentrotus lividus), bovine, hamster, rat, chicken, human, monkey, mouse, Oxyrrhis marina (dinoflagellate) |
| concentration | ~1 mg/mL |
| application(s) | immunoblotting: 0.5-1.0 μg/mL using whole extract of cultured rat adrenal pheochromocytoma (PC-12) cells |
| immunocytochemistry: suitable | |
| immunoprecipitation: suitable | |
| indirect ELISA: suitable | |
| isotype | IgG1 |
| Gene Information | human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383) |
| rat ... Tubb2(29212), Tubb2c(296554) | |
| mouse ... Tubb1(545486), Tubb2a(22151), Tubb2c(227613) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 2 |
References
| reference | Audebert, S., et al. Eur. J. Biochem. 261, 48, (1999) Abstract |
| Gingras, D., et al. Mol. Biol. Cell 9, 513, (1998) Abstract | |
| Visnjic, D., et al., Two waves of the nuclear phospholipase C activity in serum-stimulated HL-60 cells during G1 phase of the cell cycle Biochim. Biophys. Acta 1771, 514-521, (2007) Abstract | |
| Ruppert, J., and Deng, W., Gli1 acts through Snail and E-cadherin to promote nuclear signaling by β-catenin Oncogene 26, 4489-4498, (2007) Abstract | |
| Colangelo, C.M., et al., Cellular Proteins in Influenza Virus Particles PLoS Pathog. 4, e1000085, (2008) Abstract | |
| Qian Li-Wu, et al., Kaposi’s Sarcoma-Associated Herpesvirus Disrupts Adherens Junctions and Increases Endothelial Permeability by Inducing Degradation of VE-Cadherin J. Virol. 82, 11902-11912, (2008) |
